NUCLEAR LOADING CONTROL WESTERN BLOT SKIN

Mosley SR & Baker K Isolation of endogenous cytosolic DNA from cultured cells.HOTAIR modulates hepatocellular carcinoma progression by activating FUT8/core-fucosylated Hsp90/MUC1/STAT3 feedback loop via JAK1/STAT3 cascade.

NUCLEAR LOADING CONTROL WESTERN BLOT SKIN

Apoptotic extracellular vesicles are metabolized regulators nurturing the skin and hair.

Publishing research using ab133741? Please let us know so that we can cite the reference in this datasheet.Īb133741 has been referenced in 161 publications. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye ®800CW) preadsorbed ( ab216773) and Goat anti-Mouse IgG H&L (IRDye ®680RD) preadsorbed ( ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. ab133741 and Anti-GAPDH antibody - Loading Control ( ab8245) overnight at 4☌ at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Wild-type HeLa and LMNB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Loss of signal was observed when knockout cell line ab255404 (knockout cell lysate ab263825) was used. Red - Anti-GAPDH antibody - Loading Control ( ab8245) observed at 37 kDa.Īb133741 was shown to react with LMNB1 in wild-type HeLa cells in western blot. Lanes 1- 2: Merged signal (red and green). Observed band size: 66-70 kDa why is the actual band size different from the predicted? All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab133741) at 1/1000 dilution

Nuclear loading control western blot